The SenseUP-Metasensor-Technology is based on biosensors, which detect a substance of interest at molecular level and transform its concentration into graded optical signals. These signals represent a phenotype for productivity, enabling optical analysis of huge cell-libraries with literally unlimited throughput in short time.
Our biosensor-libraries hold thousands of sensor-candidates and are continuously growing. We master different sensor-concepts based on natural transcriptional-regulators, engineered transcriptional-regulators, leader-peptides, aptamers or whatever gives us the specificity and signal-output we desire.
Imagine to screen libraries with millions of variants within hours, imagine to analyse each single mutant from undirected mutagenesis experiments, each target-variant emerging from saturation- or error-prone mutagenesis, each entity in a metagenome-library... The SenseUp Metasensor-Technology enables all of that, providing strong advantages for microbial strain-development:
1. Accessing full biotechnological potential
Screening of complete libraries gives access to their full biotechnological potential, not only to fractions of it. We identify the best strain, enzyme-variant or new biocatalyst out of millions instead of thousands.
2. Natural evolution
The unlimited throughput enables natural evolution approaches. We can easily wait for the one cell under millions which accidently gained a beneficial mutation. There is no need for knowledge-based synthetic biology actions or brutal undirected mutagenesis steps. We just let nature do its job to accidently find elegant solutions for a given task (which in our case is to be selected based on strongest biosensor-response).
3. Screening from process
Screening and selection on single-cell level enables joint cultivation of millions of clones in one vessel in any scale. By that, we mimic industrial fermentation conditions as close as possible and screen from these conditions: "You get what you screen for" is a well known fact, thus screening from process-conditions gives you the strains performing best under even these industrially relevant conditions.
4. Single-cell crossbreeding
Our Single-Cell Crossbreeding-Technology links recombinase-assisted combination of natural genetic varieties to sensor-based high-throughput screening. This enables fast reproduction and combination of specific genetic compositions and direct analysis of their physiological impact under process-conditions.