Peptides & Proteins
For manufacturing of purified recombinant proteins or peptides, one has plenty of choices. However, in almost all cases one must decide between two options: Microbial systems with an inexpensive and easily scalable upstream but complex and expensive downstream processes. Or mammalian systems with a simpler downstream but costly and time-consuming upstream process.
Secretory protein/peptide production by Corynebacterium combines beneficial aspects of both worlds, an inexpensive and easily scalable upstream process and a simple and safe downstream process:
In contrast to many other microbial protein production hosts, Corynebacterium secretes only a very limited number of endogenous proteins into its culture supernatant. Moreover, it exhibits very low, if any extracellular proteolytic activity. Therefore, the secreted recombinant proteins are proteolytically stable and present in high relative purity.
Unlike E. coli or other commonly used production hosts, Corynebacterium produces no endotoxins and is generally recognized as safe (GRAS).
Using Corynes secretory capabilities, proteins are produced as active proteins, having its native conformation including complex disulfide bonds so that there is no need for denaturation and refolding steps.
SenseUp developed its Metasensor-Technology for detection of secreted recombinant proteins and peptides. Our sensors transform protein/peptide concentration at the outside of each single cell into graded optical output. This optical output indicates productivity of each single cell for a given protein/peptide, enabling unlimited screening of huge strain-libraries and application of our unique natural evolution and crossbreeding approaches to construct superior production strains.