Peptides & Proteins


For manufacturing of purified recombinant proteins or peptides, one has plenty of choices. However, in almost all cases one must decide between two options: Microbial systems with an inexpensive and scalable upstream but complex and expensive downstream process, or mammalian systems with a simpler downstream but costly and time-consuming upstream process.

Secretory protein/peptide production by Corynebacterium combines beneficial aspects of both worlds, an inexpensive and easily scalable upstream process and a simple and safe downstream process:

Advantages of Corynebacterium for expression of heterologous proteins
  • Efficient secretion of proteins (Gram positive: Single membrane barrier)

  • High stability of secreted proteins (rarely detectable protease activity)

  • Simple protein purification (only few endogenous secreted proteins)

  • Endotoxin-free and generally recognized as safe (GRAS) organism

  • Correct disulfide bond formation (folding in oxidative environment)

  • Suitable for the production of therapeutic proteins and peptides


Why Corynebacterium as additional host?
  • Complementary to E. coli physiology (Gram positive, natural secretion, suitable for peptides, suitable for inclusion body forming proteins)

  • Compatible to E. coli infrastructure (same cultivation systems, similar media and process conditions, similar handling)

  • Well established industrial application

  • Well known in feed, food and pharma-industry (easy approval)

  • Proven as efficient platform for expression/secretion of heterologous proteins



Our Metasensor-Technology, natural evolution and crossbreeding technologies are completely available for peptide or protein secreting strains based on C. glutamicum or B. subtilis.