Peptides & Proteins

For manufacturing of purified recombinant proteins or peptides, one has plenty of choices. However, in almost all cases one must decide between two options: Microbial systems with an inexpensive and scalable upstream but complex and expensive downstream process, or mammalian systems with a simpler downstream but costly and time-consuming upstream process.

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Secretory protein/peptide production by Corynebacterium combines beneficial aspects of both worlds, an inexpensive and easily scalable upstream process and a simple and safe downstream process:

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Advantages of Corynebacterium for expression of heterologous proteins

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• Efficient secretion of proteins (Gram positive: Single membrane barrier)

• High stability of secreted proteins (rarely detectable protease activity)

• Simple protein purification (only few endogenous secreted proteins)

• Endotoxin-free and generally recognized as safe (GRAS) organism

• Correct disulfide bond formation (folding in oxidative environment)

• Suitable for the production of therapeutic proteins and peptides

Why Corynebacterium as additional host?

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• Complementary to E. coli physiology (Gram positive, natural secretion, suitable for peptides, suitable for inclusion body forming proteins)

• Compatible to E. coli infrastructure (same cultivation systems, similar media and process conditions, similar handling)

• Well established industrial application

• Well known in feed, food and pharma-industry (easy approval)

• Proven as efficient platform for expression/secretion of heterologous proteins

Our Metasensor-Technology, natural evolution and crossbreeding technologies are completely available for peptide or protein secreting strains based on C. glutamicum or B. subtilis.

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