Library-Screening

With ever increasing possibilities in DNA synthesis and library-creation, the generation of millions of genetic variants, synthetic operons or completely new sequences is done within days.

But without a similarly strong screening-concept, it is impossible to access the physiological potential hidden in the sequence-space.

Our experience in flow cytometry and single cell sorting of microbial cells - with or without application of biosensors - enables screening of various libraries with literally unlimited throughput. Feel free to ask us for a tailor-made solution!

 

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Examples

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• Promoter-libraries, to identify promoters with desired properties under your individual process conditions.

• Strain-libraries, to identify the most productive strain under millions of others.

• Enzyme-libraries, to identify enzyme-variants with altered/desired catalytic activity.

• Metagenome-libraries, to identify new biocatalysts for a given reaction.

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Biosensors

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If needed, a whole bunch of sensors is ready to transfer your phenotype of interest in an optical signal:

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• Metabolite-sensors, detecting a specific metabolite such as an amino acid or other substances and transforming its concentration into a graded optical output.

• Redox-sensors, monitoring NAD(P)H/NAD(P)-balance in E. coli and by that detecting reduction-equivalent dependent biocatalytic activity.

• Secretion-sensors, detecting the concentration of a secreted peptide or protein outside the cell.

• Antibiotic activity sensors, gradually indicating the intensity of antibiotic effects on single-cell level.

• Competence sensors, monitoring competence for DNA-uptake on single-cell level.

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